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附属九院眼科范先群团队发现环状RNA促进骨修复新机制,为骨再生提供新依据
2019-09-03 浏览( 来源: 
 撰稿:许玮
 摄影:

YANKUANGGUQUESUNJIANGDAOZHIYANQIUNEIXIANHEYIWEI、SHILIXIAJIANGSHENZHISANGSHI、LUMIANJIXINGDENGYANZHONGHOUGUO。YANKUANGJIEPOUJIEGOUDETESHUXINGSHIYANKUANGGUQUESUNXIUFUDELINCHUANGXIAOGUOBUJIA。SHANGHAIJIAOTONGDAXUEYIXUEYUANFUSHUDIJIURENMINYIYUANYANKEFANXIANQUNJIAOSHOUTUANDUIZHANGQIZHILIYUYANKUANGGUXIUFUDEJICHUJILINCHUANGYANJIU,XIANGGUANYANJIUCHENGGUOCENGHUODEGUOJIAKEJIJINBUERDENGJIANG。JINQIFANXIANQUNJIAOSHOUTUANDUIYANJIUFAXIANLEYUCHENGGUFENHUAMIQIEXIANGGUANDEHUANZHUANGRNA circRNA-vgll3TONGGUOcircRNA-vgll3/miR-326-5p/ZHENGHESU(Itga5)TONGLUXIANZHUCUJINZHIFANGJIANCHONGZHIGANXIBAO(ADSCs)DECHENGGUFENHUA。CircRNA -vgll3XIUSHIDEADSCsYULINSUANGAIGUSHUINI(CPC)ZHIJIAFUHEZAIDONGWUBIAOZHUNGUQUESUNMOXINGZHONGQUDEXIANZHULIAOXIAO,ZHEIXIEYANJIUCHENGGUOJIANGWEILINCHUANGSHANGZHILIAOYANKUANGGUQUESUNTIGONGXINYIJU。GAIXIANGYANJIUCHENGGUOYIYU8YUE19RIBEIGUOJIXUESHUQIKAN《XIBAOSIWANGYUFENHUA》 (Cell Death & Differentiation)ZAIXIANFABIAO。

骨再生修复领域种子细胞问题一直未能得到很好的解决。由于来源广泛和容易获得,脂肪来源的间充质干细胞成为骨再生医学中主要的种子细胞之一,然而有限的成骨分化能力阻碍了其在骨缺损修复中的临床应用潜能。本文第一作者张丹丹博士在范先群和谷平教授的指导下,利用环状RNA显著提高了脂肪间充质干细胞的成骨分化效能。研究表明circRNAs参与决定干细胞/祖细胞的命运,但其在干细胞/祖细胞成骨中的作用与机制仍未可知。该研究发现起源于vgll3基因座的circRNA-vgll3显著促进ADSCs成骨分化,沉默circRNA-vgll3则显著降低ADSCs的成骨能力。同时,该研究发现circRNA-vgll3通过circRNA-vgll3/miR-326-5p/Itga5通路在ADSC极速飞艇s成骨分化中发挥作用:Itga5促进ADSCs成骨分化,miR-326-5p抑制Itga5翻译,circRNA-vgll3通过在细胞质中吸附miR-326-5p抑制后者活性以促进ADSCs的成骨分化。此外,该团队对circRNA-vgll3修饰的ADSCs复合CPC支架在修复大鼠标准骨缺损模型中的安全性和有效性进行了系统评价,结果表明circRNA-vgll3能增加骨矿物质密度、骨小梁数量与新骨体积,显著促进骨缺损的修复。本研究揭示了circRNA-vgll3在新骨形成中的重要作用与机制,为临床上骨缺损修复难题的解决提供了新思路。

SHANGHAIJIUYUANYANKESHIYIYANZHONGLIUYANKUANGBINGWEITESEDEZONGHEXINGYANJIUXINGXUEKE,ZAI2019NIANDUZHONGGUOYIYUANKEJILIANGZHI(STEM)ZHONGPAIMINGQUANGUODIBA。FANXIANQUNTUANDUIZAIGUOJIAGAOJISHUYANJIUFAZHANJIHUA(863JIHUA)、GUOJIAZHONGDIANYANFAJIHUADENGDEXIANGMUZHICHIXIA,JINXINGLEYANKUANGGONGNENGHUAXIUFUCAILIAODEXILIEYANJIU,BINGJIJITUIDONGXINXINGGUZHIJIACHANPINDEZHUANHUAYINGYONG;SHENRUTANTAOLEDUOZHONGZHONGZIXIBAOBAOKUOBIDOUNIANMOJIANCHONGZHIGANXIBAO、GUSUIJIANCHONGZHIGANXIBAOHEZHIFANGJIANCHONGZHIGANXIBAODENGZAIGUZAISHENGXIUFUZHONGDEZUOYONGYUJIZHI。XILIEYANJIUCHENGGUOXIANHOUFABIAOZAIAdvanced Functional Materials、Biomaterials、Chemical Engineering JournalDENGGUOJIZHIMINGXUESHUQIKAN,WEIYANKUANGGUXIUFUTIGONGLEZHASHIDELILUNHESHIYANJICHU。



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